Platelet-rich plasma (PRP) contains at least seven growth factors including epidermal growth factor, platelet-derived growth factor, transforming growth factor-beta, vascular endothelial growth factor, fibroblast growth factor, insulin-like growth factor, and keratinocyte growth factor. The therapeutic effect of PRP occurs because of the high concentration of these growth factors compared with that in normal plasma. In recent years, has been widely used across many clinical fields, especially regenerative medicine. This review will present an overview of applications of PRP in regenerative medicine. The mechanisms of PRP effects on healing also are mentioned in this review.