JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE

Nowadays magnetic nanoparticles have a great potential for biomedical applications due to their specific properties. These particles usually consist of magnetic component, often magnetite (Fe3O4) or other iron oxide, and the nonmagnetic component which ensure interaction with biological material. Magnetic particles have an important role especially for the separation of biomolecules and diagnostics, however in recent years, there are also applications of magnetic nanoparticles in the field of tissue engineering. Mesenchymal stem cells are unique especially for their ability to differentiate into many cell types and are thus very promising tool for tissue regeneration. However, there are problems with their insufficient quantity, so it is necessary to expand these cells in vitro. Herein, nanofibers were created by electrospinning from a mixture of polycaprolactone and magnetic particles (Fe3O4) at size of 50 nm. Viability and proliferation of porcine mesenchymal stem cells as well as alkaline phosphatase activity were monitored at 1st, 7th and 21st day after seeding. Adhesion and proliferation of the cells were also verified by confocal microscopy. Significantly better viability and proliferation of cells in the presence of magnetic nanoparticles were demonstrated by MTS and PicoGreen assays, respectively. These measurements also correlate with the results of confocal microscopy.

Nowadays magnetic nanoparticles have a great potential for biomedical applications due to their specific properties. These particles usually consist of magnetic component, often magnetite (Fe3O4) or other iron oxide, and the nonmagnetic component which ensure interaction with biological material. Magnetic particles have an important role especially for the separation of biomolecules and diagnostics, however in recent years, there are also applications of magnetic nanoparticles in the field of tissue engineering. Mesenchymal stem cells are uniqueespecially for their ability to differentiate into many cell types and are thus very promising tool for tissue regeneration. However, there are problems with their insufficient quantity, so it is necessary to expand these cells in vitro. Herein, nanofibers were created by electrospinning from a mixture of polycaprolactone and magnetic particles (Fe3O4) at size of 50 nm. Viability and proliferation of porcine mesenchymal stem cells as well as alkaline phosphatase activity were monitored at 1st, 7th and 21st day after seeding. Adhesion and proliferation of the cells were also verified by confocal microscopy. Significantly better viability and proliferation of cells in the presence of magnetic nanoparticles were demonstrated by MTS and PicoGreen assays, respectively. These measurements also correlate with the results of confocal microscopy.

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