Platelet Rich Plasma (PRP) and Dextrose 10% have prolife rant effect by promote growth factor so that it could be use to promote healing of soft tissue. By using PRP and dextrose 10% injection intralesion in muscle injury were hoped fasten muscle healing process and improve muscle quality. This is experimental comparative research, was done on 27 rats that divided into 3 groups. After All of subject got muscle injury grade II, The first group was administered with PRP, , the second group with dextrose 10%, the third group with NaCl 0,9% injection intralesion. After one week, the subjects were sacrificed, and their gastrocnemius muscles were examined to see the level of myoblast through immuno histochemical technique. The result show increase level of myoblast in PRP and dextrose 10% group than control, and the level of myoblast was better in PRP group than dextrose 10% group (PRP:Dextrose 10%:NaCl 0,9% = 12,33: 8,00: 5,67). In conclusion, usage PRP and dextrose 10% injection intralesion can increase level of myoblast in muscle injury grade 2, and usage PRP was better than dextrose 10%.