Comparison of different methods of centrifugation for preparation of platelet - rich plasma (PRP)

Comparison of different methods of centrifugation for preparation of platelet - rich plasma (PRP)

Abstract

Introduction: Platelet-rich plasma(PRP) has wide clinical applications which have been proved by various studies. There are various methods available now a days to prepare PRP but no standard protocol determining the time and centrifugation speed is available. This variation in the reported methods may produce samples with different platelet concentrations which may induce different clinical responses. Hence, the aim of the present study was to compare the different methods of centrifugation for preparation of platelet - rich plasma by estimating the platelet concentration in them.

Methods: Whole blood was drawn from 50 healthy volunteers into two citrate tubes which were subjected to different centrifugation speed and time. The first tube underwent double spin at 100g (soft spin) and 400g (hard spin) respectively, for 10 minutes; whereas the second tube underwent single spin at 100g for 10 minutes. The platelet counts before and after centrifugations were then compared.

Results: Single spin method yielded a plate let concentration factor of 2.19 as compared with the double spin method where, actually the platelet counts reduced (platelet concentration factor=0.83).

Conclusion: From the above findings we conclude that single centrifugation method is better than double centrifugation method in preparation of platelet rich plasma as evidenced by the high platelet concentration factor (2.19).

Abstract

Introduction: Platelet-rich plasma(PRP) has wide clinical applications which have been proved by various studies. There are various methods available now a days to prepare PRP but no standard protocol determining the time and centrifugation speed is available. This variation in the reported methods may produce samples with different platelet concentrations which may induce different clinical responses. Hence, the aim of the present study was to compare the different methods of centrifugation for preparation of platelet - rich plasma by estimating the platelet concentration in them.

Methods: Whole blood was drawn from 50 healthy volunteers into two citrate tubes which were subjected to different centrifugation speed and time. The first tube underwent double spin at 100g (soft spin) and 400g (hard spin) respectively, for 10 minutes; whereas the second tube underwent single spin at 100g for 10 minutes. The platelet counts before and after centrifugations were then compared.

Results: Single spin method yielded a plate let concentration factor of 2.19 as compared with the double spin method where, actually the platelet counts reduced (platelet concentration factor=0.83).

Conclusion: From the above findings we conclude that single centrifugation method is better than double centrifugation method in preparation of platelet rich plasma as evidenced by the high platelet concentration factor (2.19).

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