Histological Evaluation of Bone Marrow Derived Stem Cell Therapy on Experimentally Induced Osteoarthritis in Albino Rats' Knee Joint

Histological Evaluation of Bone Marrow Derived Stem Cell Therapy on Experimentally Induced Osteoarthritis in Albino Rats' Knee Joint

Abstract:

Aim of work: This work aims to evaluate the efficacy of intra-articular injection of bone marrow derived-mesenchymal stem cells (MSCs) in treatment of mono-iodoacetate (MIA) induced osteoarthritis in rat knee joint monitored by histological and immunohistochemical methods. Material and methods: this study was carried out on 45 adult male albino rats. They were classified into 4 groups: group I (control group), group II (osteoarthritic group) in which rats received 1 mg of MIA and sacrificed after 2 weeks and after 4 weeks, group III (stem cell treated group) in which rats received MSCs 2 weeks after MIA injection or 4 weeks after MIA and sacrificed 2 weeks later and group IV (untreated group) in which rats received PBS 2 weeks after MIA injection or 4 weeks after MIA and sacrificed 2 weeks later. Sections were taken from rats' knee joints and stained with Hematoxylin and Eosin, toluidine blue, immunohistochemichal stains for collagen type II. Sections were examined by light microscopy. The mean articular cartilage (AC) thickness, optical density of cartilage matrix proteoglycan and area percent of collagen type II immunoreactivity were measured using image analyzer and statistically analyzed.

Abstract:

Aim of work: This work aims to evaluate the efficacy of intra-articular injection of bone marrow derived-mesenchymal stem cells (MSCs) in treatment of mono-iodoacetate (MIA) induced osteoarthritis in rat knee joint monitored by histological and immunohistochemical methods. Material and methods: this study was carried out on 45 adult male albino rats. They were classified into 4 groups: group I (control group), group II (osteoarthritic group) in which rats received 1 mg of MIA and sacrificed after 2 weeks and after 4 weeks, group III (stem cell treated group) in which rats received MSCs 2 weeks after MIA injection or 4 weeks after MIA and sacrificed 2 weeks later and group IV (untreated group) in which rats received PBS 2 weeks after MIA injection or 4 weeks after MIA and sacrificed 2 weeks later. Sections were taken from rats' knee joints and stained with Hematoxylin and Eosin, toluidine blue, immunohistochemichal stains for collagen type II. Sections were examined by light microscopy. The mean articular cartilage (AC) thickness, optical density of cartilage matrix proteoglycan and area percent of collagen type II immunoreactivity were measured using image analyzer and statistically analyzed.

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